The physiological differences and chlorophyll content of lettuce grown under three different light treatments (fluorescent, F3 LED, and X5 LED) were investigated. These experiments were conducted in a vertical growing system on two lettuce varieties (Red Oakleaf and Green Butter Salanova) under the three different light treatments with all other environmental parameters (including wattage) kept constant. The fluorescent-grown lettuce had thinner leafs and cores and less leaf color (anthocyanin) than the LED-grown lettuce. Chlorophyll extraction confirmed that chlorophyll a and b to dry weight increased, and the ratio of chlorophyll a/b decreased in the fluorescent-grown lettuce. These results are an indication of thinner cell walls and enlargement of photosynthetic antennae systems in the fluorescent-grown lettuce compared to the LED-grown lettuce. The difference in chlorophyll a/b ratio for red and green lettuce under the different LED spectrums suggest green lettuce grew equally well under both LED spectrums and red lettuce favored the F3 over the X5 spectrum.
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